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Image Search Results
Journal: Translational Psychiatry
Article Title: Music prevents stress-induced depression and anxiety-like behavior in mice
doi: 10.1038/s41398-023-02606-z
Figure Lengend Snippet: A – L Music prevents abnormal expression of oxidative stress-related factors such as NO, SOD, GSH, MDA, CAT, T-AOC in the blood or hippocampus and prefrontal cortex of CUMS. All values are presented as means ± SEM. n = 6 per group. * (#) P < 0.05; ** (##) P < 0.01; *** (###) P < 0.001. NO nitric oxide, SOD superoxide dismutase, GSH glutathione peroxidase, MDA malondialdehyde, CAT catalase, T-AOC total antioxidant capacity.
Article Snippet: The levels of malondialdehyde (MDA), glutathione peroxidase (GSH-Px), catalase (CAT), nitric oxide (NO),
Techniques: Expressing
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway
doi: 10.1155/2020/5954635
Figure Lengend Snippet: The schematic illustration representing the whole experiment. SSCs: spermatogonial stem cells; ROS: reactive oxygen species; MDA: malondialdehyde; T-AOC: total antioxidant capacity; SOD: superoxide dismutase; CAT: catalase; GSH-Px: glutathione peroxidase; TEM: transmission electron microscopy.
Article Snippet: The T-AOC of SSCs (10 6 cells per sample) was measured using the Total
Techniques: Transmission Assay, Electron Microscopy
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway
doi: 10.1155/2020/5954635
Figure Lengend Snippet: Effects of different melatonin concentrations on the malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) of spermatogonial stem cells (SSCs) during cryopreservation. (a) MDA content in different groups. (b) T-AOC in different groups. Different superscript letters (A–C) indicate significant differences ( P < 0.05). Values are expressed as the means ± SD ( n = 3).
Article Snippet: The T-AOC of SSCs (10 6 cells per sample) was measured using the Total
Techniques:
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway
doi: 10.1155/2020/5954635
Figure Lengend Snippet: Effects of different melatonin concentrations on the antioxidant enzyme activity of spermatogonial stem cells (SSCs) during cryopreservation. (a) Superoxide dismutase (SOD) activity. (b) Catalase (CAT) activity. (c) Reduced glutathione (GSH) activity. (d) Oxidized glutathione (GSSG) activity. (e) GSH/GSSG ratio, which is an indirect indicator of glutathione peroxidase (GSH-Px) activity. Different superscript letters (A–C) indicate significant differences ( P < 0.05). Values are expressed as the means ± SD ( n = 3).
Article Snippet: The T-AOC of SSCs (10 6 cells per sample) was measured using the Total
Techniques: Activity Assay
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway
doi: 10.1155/2020/5954635
Figure Lengend Snippet: Antioxidant enzyme proteins in frozen-thawed goat spermatogonial stem cells (SSCs) detected by western blotting. (a) Western blot image of catalase (CAT), glutathione peroxidase 5 (Gpx5), superoxide dismutase 2 (SOD2), and SOD1. (b–e) Relative protein expression in the control and 10 −6 M melatonin treatment group. The superscript ∗ indicates a significant difference at P < 0.05, and the superscript ∗∗ indicates a significant difference at P < 0.01. The values are expressed as the means ± SD ( n = 3). Mel: 10 −6 M melatonin.
Article Snippet: The T-AOC of SSCs (10 6 cells per sample) was measured using the Total
Techniques: Western Blot, Expressing, Control
Journal: Stem Cell Research & Therapy
Article Title: Potential effect of amniotic fluid-derived stem cells on hyperoxia-induced pulmonary alveolar injury
doi: 10.1186/s13287-022-02821-3
Figure Lengend Snippet: Effect of stem cell treatment on biochemical markers. A Western blot analyses of IL-6 and RhoA in lung tissue in all groups. β actin was used for normalization. Images of the bands where obtained from the same gel with no cropping in between the bands. Full length blots are included in the Additional file Fig. S1. B , C Representative graphs for relative expression of IL-6 and RhoA respectively. D , E Representative graphs for quantification of MDA and TAC in lung tissue of all experimental groups. Data is represented in mean. n = 3 in all groups. a; significant compared to CG. b: significant compared to HG. c: significant compared to CFM-G. d: significant compared to SC-PG. e: significant compared to SC-TG. Pairwise comparison bet. Each 2 groups using Post Hoc Test (Tukey). Significance at p ≤ 0.05. Error bars represent S.E.M. Stem cell treatment significantly reduced IL-6, RhoA and increased TAC, while stem cell prophylaxis significantly reduced MDA. Abbreviations: CG; control group, HG; Hyperoxia group, CFM-G; Cell-free media-treated group, SC-PG; Stem cells-prophylactic group, SC-TG; Stem cells-treated group, Unt-G Untreated group, IL-6; interleukin 6, RhoA; Ras homolog family member A, MDA; malondialdehyde, TAC; total antioxidant capacity
Article Snippet: The lung tissues were homogenized in 1–2 ml of 5 mM cold potassium phosphate buffer (pH 7.4), centrifuged at 4000 rpm for 15 min at 4 °C and the supernatant was removed and stored at − 80 °C for assay of total
Techniques: Western Blot, Expressing, Comparison, Control